econotaq dna polymerase (Lucigen Corp)

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Lucigen Corp econotaq dna polymerase
Econotaq Dna Polymerase, supplied by Lucigen Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/econotaq/dna+econotaq+polymerase/pm42234578-64-21-24
Average 86 stars, based on 1 article reviews

econotaq dna polymerase - by Bioz Stars, 2026-10

86/100 stars

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Polymerase Chain Reaction:

Article Title: Convergent Clonal Evolution of Signaling Gene Mutations Is a Hallmark of Myelodysplastic Syndrome Progression
Article Snippet: .. EconoTaq (Lucigen) was used for all PCR reactions. .. Amplicons from individual reactions were pooled to create libraries that were sequenced on the Illumina MiSeq.

Article Title: In vivo protein N-acylation
Article Snippet: .. Subsequently, PCR amplification using the appropriate oligos was performed to add −50 bp flanking homology sequences to target specific genes (arsB known neutral site for chromosomal integration or ompT a membrane associated protease) using Econotaq (Lucigen Middleton, WI) according to manufacturer's instructions, with an initial 10 minutes denaturation at 94°, followed by 35 cycles of 94°, for 15 seconds, 52° for 15 seconds, and 72° for 5 minutes. .. Cassettes for “curing” the tet-sacB cassette and both simultaneous arsB deletion and integration of a low phosphate inducible his-tagged ulp1hydrolase (codon optimized for E. coli) or deletion of ompT were obtained as GblocksTM from IDT.

Article Title: G-Protein-Coupled Receptor Kinase 2 Limits CCL21-Induced T Cell Migration via Phospholipase Cγ1
Article Snippet: RNA was digested using RNAse H (#M0297; NEB, Ipswich, MA, USA). .. PCR was performed using EconoTaq plus (#30033–1; Lucigen, Middleton, WI, USA) with 94 °C denaturation for 3 min, followed by 35 cycles at 94 °C for 30 s, 62 °C for 30 s, and 72 °C for 1 min. GRK2 primers (Sigma Aldrich, St. Louis, MO, USA) (Forward: 5-CACCATCAACGCTGAGACAG-3, Reverse: 5-ATCTTGAGGAGCAGGCACTT-3) and as an internal control, β-actin primers (Forward: 5-GTGGCATCCACGAAACTACC-3, Reverse: 5-CCTGCTTGCTGATCCACATC-3) were used. ..

Article Title: G-Protein-Coupled Receptor Kinase 2 Limits CCL21-Induced T Cell Migration via Phospholipase Cγ1
Article Snippet: RNA was digested using RNAse H (#M0297; NEB, Ipswich, MA, USA). .. PCR was performed using EconoTaq plus (#30033-1; Lucigen, Middleton, WI, USA) with 94 ◦C denaturation for 3 min, followed by 35 cycles at 94 ◦C for 30 s, 62 ◦C for 30 s, and 72 ◦C for 1 min. GRK2 primers (Sigma Aldrich, St. Louis, MO, USA) (Forward: 5-CACCATCAACGCTGAGACAG-3, Reverse: 5-ATCTTGAGGAGCAGGCACTT-3) and as an internal control, β-actin primers (Forward: 5-GTGGCATCCACGAAACTACC-3, Reverse: 5-CCTGCTTGCTGATCCACATC3) were used. ..

Selection:

Article Title: Compositions and methods for metabolic control of a biofermentation process with synthetic metabolic valves
Article Snippet: .. Briefly, the tet-sacB selection/counterselection cassette was amplified using the appropriate oligos supplying ̃50 bp flanking homology sequences using Econotaq (Lucigen Middleton, Wis.) according to manufacturer's instructions, with an initial 10 minutes denaturation at 94° C., followed by 35 cycles of 94° C., for 15 seconds, 52° C. for 15 seconds, and 72° C. for 5 minutes. .. Cassettes used for “curing” of the tet-sacB cassette or direct integration (when an antibiotic marker is present) were obtained as gBlocks from IDT.

Article Title: Compositions and methods for metabolic control of a biofermentation process with synthetic metabolic valves
Article Snippet: .. Briefly, the tet-sacB selection/counterselection cassette was amplified using the appropriate oligos supplying ̃50 bp flanking homology sequences using Econotaq (Lucigen Middleton, WI) according to manufacturer's instructions, with an initial 10 minutes denaturation at 94° C., followed by 35 cycles of 94° C., for 15 seconds, 52° C. for 15 seconds, and 72° C. for 5 minutes. .. Cassettes used for “curing” of the tet-sacB cassette or direct integration (when an antibiotic marker is present) were obtained as gBlocks from IDT.

Article Title: Compositions, systems, and methods for high level expression of recombinant protein
Article Snippet: .. Briefly, the tet-sacB selection/counterselection cassette was amplified using the appropriate oligos supplying ̃50 bp flanking homology sequences using Econotaq (Lucigen Middleton, WI) according to manufacturer's instructions, with an initial 10 minutes denaturation at 94° C., followed by 35 cycles of 94° C., for 15 seconds, 52° C. for 15 seconds, and 72° C. for 5 minutes. .. Cassettes used for “curing” of the tet-sacB cassette were obtained as gBlocks from (Integrated DNA Technologies, Coralville, Iowa, USA).

Amplification:

Article Title: Compositions and methods for metabolic control of a biofermentation process with synthetic metabolic valves
Article Snippet: .. Briefly, the tet-sacB selection/counterselection cassette was amplified using the appropriate oligos supplying ̃50 bp flanking homology sequences using Econotaq (Lucigen Middleton, WI) according to manufacturer's instructions, with an initial 10 minutes denaturation at 94° C., followed by 35 cycles of 94° C., for 15 seconds, 52° C. for 15 seconds, and 72° C. for 5 minutes. .. Cassettes used for “curing” of the tet-sacB cassette or direct integration (when an antibiotic marker is present) were obtained as gBlocks from IDT.

Article Title: Evolution of major flowering pathway integrators in Orchidaceae.
Article Snippet: .. The amplification reactions of each GOI were done using 6.0 μl of EconoTaq (Lucigen, Middleton, USA), 4 μl of nuclease-free water, 1 μl of bovine serum albumin (5 μg/ml), 1 μl of Betaine (5 μg/ml), 1 μl of forward primer, 1 μl of reverse primer, and 1 μl of cDNA, for a total of 15 μl. ..

Membrane:

Control:

Article Title: G-Protein-Coupled Receptor Kinase 2 Limits CCL21-Induced T Cell Migration via Phospholipase Cγ1
Article Snippet: RNA was digested using RNAse H (#M0297; NEB, Ipswich, MA, USA). .. PCR was performed using EconoTaq plus (#30033-1; Lucigen, Middleton, WI, USA) with 94 ◦C denaturation for 3 min, followed by 35 cycles at 94 ◦C for 30 s, 62 ◦C for 30 s, and 72 ◦C for 1 min. GRK2 primers (Sigma Aldrich, St. Louis, MO, USA) (Forward: 5-CACCATCAACGCTGAGACAG-3, Reverse: 5-ATCTTGAGGAGCAGGCACTT-3) and as an internal control, β-actin primers (Forward: 5-GTGGCATCCACGAAACTACC-3, Reverse: 5-CCTGCTTGCTGATCCACATC3) were used. ..

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